Fluorescence is a well-known phenomenon with many practical applications that has been studied for decades. Despite this, a ...

2 These modifications have tremendously improved this technique's sensitivity and specificity. Besides tweaking the original ...

In biology, stochastic optical reconstruction microscopy — a technique that uses single-molecule detection and photo-switchable probes to achieve high-resolution fluorescence imaging — now ...

Thus, wide-field fluorescence imaging, a technique that captures autofluorescence generated primarily in the stroma, may give rise to a loss of fluorescence in both benign and precancerous lesions.

An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic ... and the physics of scattering and fluorescence. With a wealth of ...

Researchers are exploring the use of adaptive optics to correct for aberrations in thick samples, as well as the integration of STED with other super-resolution techniques like single-molecule ...

but the technique requires subjective interpretation and depends on the visual recognition skills of the examiner. Capturing and analyzing digital fluorescence images can reduce subjectivity and ...

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For human researchers, it takes many years of work to discover new super-resolution microscopy techniques. The number of possible optical configurations of a microscope -- for example, where to place ...

As the electrons interact with the specimen, they are scattered or transmitted, producing an image that is magnified and focused onto an imaging device, such as a fluorescent screen ... Scanning ...

The optical microscope technique usually includes bright-field, dark-field, and fluorescence imaging, which are commonly based on cumbersome optical components. Especially for fluorescence and ...