Early technologies to globally map phosphorylation were aimed at identifying novel kinase–substrate relationships. These technologies took the strategy of increasing the throughput of in vitro ...

Thus, strains containing the mutated kinase or substrate alone were viable ... to develop new technology aimed at globally mapping phosphorylation in eukaryotes. A number of technologies have ...

Phosphorylation is the most ubiquitous modification found in cells. Enzymes that catalyze the reaction of transferring phosphate groups to proteins using adenosine triphosphate as a substrate are ...

The reaction catalyzed by succinyl-CoA synthetase (in which GTP synthesis occurs) is an example of substrate-level phosphorylation.

When a site-specific protease is added to the phosphorylation reaction, it cleaves nonphosphorylated peptides causing them to lose the FRET signal. “We have developed a portfolio of substrates ...