Protein phosphorylation is a covalent ... Original research articles describing the motif are linked to each motif. These include 24 phosphatase substrate motifs and 80 motifs that bind to domains ...

Early technologies to globally map phosphorylation were aimed at identifying novel kinase–substrate relationships. These technologies took the strategy of increasing the throughput of in vitro ...

Thus, strains containing the mutated kinase or substrate alone were viable ... to develop new technology aimed at globally mapping phosphorylation in eukaryotes. A number of technologies have ...

When a site-specific protease is added to the phosphorylation reaction, it cleaves nonphosphorylated peptides causing them to lose the FRET signal. “We have developed a portfolio of substrates ...

Phosphorylation is the most ubiquitous modification found in cells. Enzymes that catalyze the reaction of transferring phosphate groups to proteins using adenosine triphosphate as a substrate are ...