Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using ...

Before sectioning, the blocks in the soaking medium, if such is used, are cooled to approximately 15°C. Better than average sections are obtained.

Proper sample preparation is crucial for successful ultramicrotomy. The sample must be embedded in a suitable resin that provides support and stability during sectioning. Common embedding resins ...

Be advised that it is very difficult to trim and embed tissues so precisely that it can be determined before sectioning which sections will contain such areas. In such cases it is advisable to request ...