RNA splicing is a modification of the nascent pre-messenger RNA (pre-mRNA) transcript in which introns are removed and exons are joined prior to translation. For many eukaryotic introns ...
Transfer 400 μl of the oil-surfactant mixture to a CryoTube vial, and add a 3 × 8 mm stir bar. Begin stirring the mixture at 1,000 r.p.m. on the magnetic stirrer. Failure to add BSA or another ...