Imaging of macrophage mitochondria dynamics in vivo reveals …
2020年2月3日 — Using confocal microscopy, structured illumination microscopy (SIM) and transmission electron microscopy (TEM), we found that M1 and M2b macrophages had highly fragmented and discrete mitochondria, while M2a and M2c macrophages had elongated and interconnected ones (Figure (Figure2E, 2 E, 2G and 3A).
Macrophages use different internalization mechanisms to clear apoptotic ...
2006年4月21日 — Scanning electron microscopy (SEM) revealed that apoptotic bodies were taken up by macrophages with formation of tight fitting phagosomes, similar to the ‘zipper’-like mechanism of...
Discovering Macrophage Functions Using In Vivo Optical Imaging ...
2018年3月15日 — In this article, we will review the advances in optical imaging techniques for macrophage tracking and analysis in vivo, specifically covering examples to show how multiple imaging modalities help us to track and resolve their dynamics in vivo under disease contexts.
Tissue-infiltrating macrophages mediate an exosome-based …
2020年1月2日 — Electron microscopy revealed the presence of EVs in the media of Er1 F/− macrophages with a size ranging between 30 and 80 nm; the latter corresponds to that known for exosomes (40–100 nm ...
Serine synthesis controls mitochondrial biogenesis in macrophages …
2024年5月17日 — Increasing mitochondrial biogenesis or restoring mitochondrial integrity alleviates macrophage-mediated inflammatory responses by down-regulation of interleukin-1β (IL-1β) and tumor necrosis factor–α (TNF-α) secretion (8).
Morphologic, phenotypic, and transcriptomic characterization of ...
2017年8月17日 — Scanning electron microscopy revealed distinct morphologies of polarized macrophages with formation of multinucleated cells in M2-macrophages, while immunofluorescence employing literature-based prototype-antibodies against CD16, CD32, iNOS, MHC class II (M1-markers), CD163, CD206, and arginase-1 (M2 …
A Network of Macrophages Supports Mitochondrial ... - Cell Press
Interestingly, although many mitochondrial proteins were less represented after cMac-depletion (Figure S1H), transmission electron microscopy (TEM) imaging revealed a significant increase in the number of mitochondria within cardiomyocytes, which we confirmed by immunostaining for mitochondrial protein Tom20 in heart sections (Figures 1E, S1I ...
Morphologic, phenotypic, and transcriptomic characterization of
2017年8月17日 — Scanning electron microscopy revealed distinct morphologies of polarized macrophages with formation of multinucleated cells in M2-macrophages, while immunofluorescence employing literature-based prototype-antibodies against CD16, CD32, iNOS, MHC class II (M1-markers), CD163, CD206, and arginase-1 (M2 …
Classification of M1/M2-polarized human macrophages by label …
2017年8月21日 — Human monocyte derived macrophages have been investigated using hyperspectral reflectance confocal microscopy, and hyperspectral datasets have been analysed in terms of M1 vs. M2 polarization...
Macrophage Membranes Viewed through a Scanning Electron Microscope …
When rabbit peritoneal macrophages were cultured in serum and Tyrode medium, numerous membranous structures varying in size and transparency were observed to cover the surface in complex cascading patterns. Modifications in the methods of fixation and drying probably accounted for the new perspective of macrophage surfaces gained in this study.