number of 293T cells at 60-70% confluency - Cell Biology
网页I have a chart which states that for a 10cm plate - fully confluent, there are 8.8 million cells. However, this is true for Hela cells. So I'm not sure if these figures also applicable for 293 cells. In a confluent flask 75cm2 with 293FT cells, there will be approx. 30 million cells. I use 60mm dish, the number of 293T at 70%confluence is about ...
HEK 293T cells - problems getting proper attachment.
网页2008年6月9日 · Any harsh handling will make the cells to come off. I grow them in DMEM+10%FBS, in Greiner flasks. Add PBS on sides of the flask tilt flask gently twice, aspirate off and trypsinize for 2 min, and quench with media. I have not faced 'attaching' problem even when I grow them in 35mm, 60mm and 90mm dishes for transfections.
passaging 293T cells - Cell Biology - protocol-online.org
网页i usually split 293T 1:10 every 4th day. add 1ml trypsin, just like you, then tap the plate for all the cells to come off the bottom. then add 10 ml of medium, pipet a lot - no clumps or other stuff, and then take 1 ml and spread drop by drop all over the surface of the medium on fresh plate. then i shake the plate in all directions like million times then carefully put …
average total protein content per 293t cell - (Aug/04/2010 )
网页2010年8月5日 · Basically, i never worked with mammalian cell lines and i need to express a tagged protein in 293t cells and have approximately 1 to 5mg of total protein (which i will latter on affinity purify). Some literature mention they start from 20 transfecetd 10cm dishes with 20% confluent cells (i don't know why they tarsnfect 20% confluent cells). I ...
what is the difference of 293T, HEK293 and HEK293T?
网页2005年9月14日 · 293 and HEK293 cells are same. 293T and HEK293T cells are same. 293T cells are 293 cells transformed by expression of the large T antigen from SV40 virus that inactivates pRb. you can get info on large T here. Fred.
HEK 293 or 293T for lentivirus - Cell Biology
网页2006年7月31日 · They have higher energy needs than most other cell lines, and the standard media formulation can be found at the Invitrogen web site for their version of these cells. Invitrogen recommends their cells 293FT for lentivirus. I compared 293T and 293FT's didnt find much of a difference in viral titres. I use DMEM- 10%FBS, + L-GLn and 1% P/S …
HEK293 cells Methods, Protocols and Troubleshootings
网页HEK293T and HEK293 - (reply: 4) problems with 293T cells after medium removal!!! - (reply: 6) establishment of stable cell line using hek293t - (reply: 2) Help!my 293T stop growth and become slim 24h after 1:3 splitting - unless you change new medium for them 24h after splitting (reply: 3)
293t Cell Attachment Issues - Tissue and Cell Culture - Protocol …
网页2008年10月16日 · DO not add anything on cells directly. You can completely tilt the flask 90 degrees and add PBS on sides, gently rock twice and discard. 2 minutes of incubation of trypsin at 37 will detach all cells. 293Ts are very finicky so treat them very tenderly. -Calvin*-. I'm solving my problem as I'm working through the project.
Are there TGFB receptors in 293T cells? - Cell Biology
网页2006年11月1日 · This is a long shot, but does anybody know off the top of their head whether 293T cells (a common human cell line) typically express TGFB (transforming growth factor beta) receptors? What would be the simplest way to find out this information?
How many cells to seed? - Tissue and Cell Culture - Protocol Online
网页2010年4月8日 · This recommends between 1 x 10^5 to 3 x 10^5 cells for one well of a 6-well dish to transfect the following day - so scaled up you'd be aiming for a ballpark figure of 1 x 10^6 cells for a 10cm plate. This works fine for most cell types (including HeLas), however 293Ts are very small so if you want them ready to transfect the following day I ...