Biosynthesis of cannabigerol and cannabigerolic acid: the …
2023年5月27日 · We identified an aromatic prenyltransferase homolog from Aspergillus terreus (AtaPT) that uses GPP to prenylate OA and olivetol to CBGA and CBG, respectively. To optimize cannabinoid production, AtaPT variants were generated to improve substrate binding.
Foldseek reveals a CBGA prenylating enzyme GlyMa
2024年2月12日 · An enzyme derived from Glycine max known as GLYMA_02G168000, which is a predicted homogentisate solanyltransferase, was identified and found to have affinity for the prenylation of geranyldiphosphate (GPP) and olivetolic acid (OA) to produce cannabigerolic acid (CBGA) and cannabigerol (CBG).
Foldseek reveals a CBGA prenylating enzyme GlyMa_02G168000
2024年2月12日 · An enzyme derived from Glycine max known as GLYMA_02G168000, which is a predicted homogentisate solanyltransferase, was identified and found to have affinity for the prenylation of geranyldiphosphate (GPP) and olivetolic acid (OA) to produce cannabigerolic acid (CBGA) and cannabigerol (CBG).
Hormonal control of promoter activities of Cannabis sativa ...
2023年5月27日 · Cannabis sativa aromatic prenyltransferase 4 (CsPT4) and 1 (CsPT1) have been shown to catalyze cannabigerolic acid (CBGA) biosynthesis, a step that rate-limits the cannabinoid biosynthetic...
Catalytic Potential of Cannabis Prenyltransferase to Expand …
2023年11月27日 · Biologically active cannabinoids are derived from cannabigerolic acid (CBGA), which is biosynthesized by aromatic prenyltransferase CsPT4. We exploit the catalytic versatility of CsPT4 to synthesize various CBGA analogues, including a geranylated bibenzyl acid, the precursor to bibenzyl cannabinoids of liverwort origin.
(PDF) Biosynthesis of cannabigerol and cannabigerolic acid: the ...
2023年5月27日 · Herein, we describe the identification and repurposing of aromatic prenyltransferase (AtaPT), which when coupled with native enzymes of C. sativa can form an Escherichia coli production system...
<br>Foldseek 揭示了来自 Glycine max 的 CBGA 异戊二烯化酶 …
一种源自 大豆的 酶，称为 GLYMA_02G168000，是一种预测的尿黑酸茄基转移酶，经鉴定并发现对香叶基二磷酸 (GPP) 和橄榄酸 (OA) 的异戊二烯化具有亲和力，可产生大麻酚酸 (CBGA) 和大麻酚 (CBG) 。 CBGA 的 体外 生产是通过在 酿酒酵母 中异源表达异戊烯基转移酶来完成的。 酵母细胞生长后，收获了纯化的微粒体部分，其中富含膜结合异戊二烯基转移酶 GlyMa_02G168000。 将纯化的微粒体部分添加到反应基质中促进了外部提供的 OA 与 GPP 的成功异戊二烯化，最终 …
2023年5月27日 · we describe the identication and repurposing of aromatic prenyltransferase (AtaPT), which when coupled with native enzymes of C. sativa can form an Escherichia coli production system for CBGA in cell lysates and CBG in whole cells.
Rational Design and Modification of NphB for Cannabinoids …
The prenyltransferase (NphB) is the first step in combining geranyl diphosphate (GPP) and olivetolic acid (oa) to produce cannabigerolic acid (CBGA) . NphB is capable of catalyzing the C- and O-geranylation of a variety of aromatic compounds [24,25].
Synthetic Pathways to Non-Psychotropic Phytocannabinoids as
2023年7月5日 · Then, aromatic prenyltransferase inserts the prenyl group at the highly nucleophilic 2-resorcinol position to provide cannabigerolic acid (CBGA) . Then, while CBGA provides CBG upon a non-enzymatic loss of CO 2 , through reactions catalyzed by the opportune synthases, it provides the cannabinolic acids CBDA, and CBCA, which in turn provide the ...