A Guide to Fluorescence Lifetime Imaging Microscopy (FLIM)
2022年7月21日 · Fluorescence lifetime imaging microscopy (FLIM) exploits the inherent properties of fluorescent dyes to produce an image. Fluorescent molecules have become essential tools for cell biologists. They are widely used in microscopy to visualize many different structures, targets and dynamic processes within cells, tissues, and even whole organisms.
Introduction to Widefield Microscopy - Leica Microsystems
2017年6月29日 · The main advantages of confocal microscopy are that user-defined regions of interest can be selected, negating the need for the entire specimen to be exposed to a fluorescent light source. In addition, the confocal microscope can be used to obtain optical sections through a specimen which can have the advantage of excluding much of the out-of ...
How to Prepare your Specimen for Immunofluorescence Microscopy
2022年1月10日 · Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence, TIRF, GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research …
Fluorescence in Microscopy | Science Lab | Leica Microsystems
2011年4月27日 · Fluorescence microscopy is a special form of light microscopy. It uses the ability of fluorochromes to emit light after being excited with light of a certain wavelength. Proteins of interest can be marked with such fluorochromes via antibody staining or …
Total Internal Reflection Fluorescence (TIRF) Microscopy
2015年3月2日 · Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy delivers images with an outstandingly high axial resolution below 100 nm. This allows the observation of membrane-associated processes.
Fluorescence | Applications - Leica Microsystems
Find out how fluorescence microscopes from Leica Microsystems support your research. Fluorescence is one of the most commonly used physical phenomena in biological and analytical microscopy for its high sensitivity and high specificity. Fluorescence is a form of luminescence that through microscopy allows users to determine the distribution of a single molecule species, its …
Fluorescent Dyes | Science Lab | Leica Microsystems
2022年1月10日 · A basic principle in fluorescence microscopy is the highly specific visualization of cellular components with the help of a fluorescent agent. This can be a fluorescent protein – for example GFP – genetically linked to the protein of interest. If cloning is impossible – for instance in histologic samples – techniques such as immunofluorescence staining are used to visualize …
An Introduction to Fluorescence | Science Lab - Leica Microsystems
2023年6月2日 · For microscopy, fluorescence is the most useful kind of luminescence. Fluorochromes can easily be excited with their specific wavelength via certain light sources, e.g., lamps and filter systems or lasers, and the emitted light can be distinguished from the excitation light by the wavelength (Stokes’ shift).
Handbook of Optical Filters for Fluorescence Microscopy
2013年8月6日 · Fluorescence microscopy and other light-based applications require optical filters that have demanding spectral and physical characteristics. Often, these characteristics are application-specific and an optic that might be appropriate and optimal for one is both inappropriate and sub-optimal for another.
Epi-Illumination Fluorescence and Reflection-Contrast Microscopy ...
2023年12月14日 · The development of epi-illumination microscopy by Leitz also led to a further development: reflection-contrast microscopy (RCM) . Reflection-contrast microscopy provides strong signals from specimens stained with routine absorbing immunomarkers, such as peroxidase-DAB, immunogold-silver, and immuno-phosphatase (Figures 10 and 11).