Gateway Cloning Protocols | Thermo Fisher Scientific - US
LR reaction. Transferring your gene from a Gateway entry clone to destination vector is an easy 1 hour reaction. See below for an overview of the set-up. For more detailed information, refer to the manual. Add the following components to a 1.5 ml tube at room temperature and mix: Entry clone (50-150 ng) 1–7 µl
分子克隆——Gateway 克隆 - 知乎 - 知乎专栏
2021年12月12日 · 如今我们在克隆时，不再只有传统的 限制酶 克隆一种选择，相反，还有许多其它的 分子克隆技术 ，它们可以在特定的资源、时间、实验需要下有效工作。 自从1990s发现以来，Gateway克隆技术作为一种可以快速、高效将DNA序列转移到载体上的克隆方法流行开来。
分子克隆技术-Gateway 克隆（LR 反应）-丁香实验
2023年4月27日 · Gateway 克隆原理基于不同酶的催化反应，能够快速、准确地完成 DNA 序列的切割、转录和接合。其中 LR 反应发生在入门载体的 attL 位点和目标载体的 attR 位点之间，该反应由 LR 克隆酶混合物催化，产生了两侧具有 attB 位点的目的 DNA 表达载体。
Gateway克隆技术原理-gateway克隆实验流程 -赛默飞 | Thermo …
lr 反应 将您的基因从 Gateway 入门克隆转移到目标载体的过程是一个很简单的反应，仅需 1 个小时。 请参阅下面的设置概述。
Gateway Cloning Technique - SnapGene
The first step—a BP reaction—creates an entry clone containing the DNA insert flanked by two attL sites. The second step—an LR reaction—creates an expression clone containing the DNA insert flanked by two attB sites. Multisite Gateway cloning allows up to four fragments to be inserted simultaneously. How does Gateway Cloning work?
Gateway™ LR Clonase™ II Enzyme mix - Thermo Fisher Scientific
Gateway™ LR Clonase™ II enzyme mix catalyzes the in vitro recombination between an entry clone (containing a gene of interest flanked by att L sites) and a destination vector (containing att R sites) to generate an expression clone.
Plasmids 101: Gateway Cloning - Addgene
The LR Reaction takes place between the att L sites of the generated entry clone and the att R sites of the destination vector. This reaction is catalyzed by the LR Clonase enzyme mix. As a result, an expression clone with the DNA of interest flanked by att B sites is generated.
BP反应-构建入门载体&LR反应-构建表达载体 - 知乎
该反应由 LR Clonase 酶混合物介导，利用蛋白机制从入门克隆中切除目的基因，整合至目标载体中，然后用作您的表达克隆。 其逆反应很简单：利用 BP Clonase 酶混合物进行 BP 反应（attB 和 atPP 位点之间的重组）。
Gateway® LR ClonaseTM II enzyme mix is a proprietary enzyme and buffer formulation containing the bacteriophage lambda recombination proteins Integrase (Int) and Excisionase (Xis), the E. coli-encoded protein Integration Host Factor (IHF) (1), and reaction buffer provided in a single mix for convenient reaction set up.
LR Reaction for expression clone (total volume 5 ul) : 1. Add following components to a 1.5 ml microcentrifuge tube, vortex briefly twice. Spin down by a microcentrifuge briefly.