Nitroxide spin labels and EPR spectroscopy: A powerful …
2021年7月1日 · In particular, continuous-wave EPR (cw-EPR) spectra can detect the local conformational dynamics for proteins under various conditions. Moreover, pulse-EPR experiments on doubly spin-labelled proteins allow measuring distances between spin centres in the 1.5–8 nm range, providing information about structures and functions.
Analyzing CW EPR Spectra of Nitroxide Labeled Macromolecules
2023年9月7日 · Given an EPR spectrum of a nitroxide attached to a protein, typically a lot of metadata is known to aid in selecting an appropriate model. This includes the spin label chemistry, the type of protein (size, water soluble, membrane, etc.), its environment (buffer, detergent, lipids, etc.), and the experimental conditions (temperature, pressure ...
Nitroxide Spin Labels—Magnetic Parameters and Hydrogen
2018年10月3日 · In this work, we report on a detailed high-field EPR investigation of magnetic parameters of deuterated nitroxide radicals dissolved in deuterated frozen solvents of polar or unpolar, protic or aprotic character including ortho -terphenyl, methanol, propanol, glycerol, aniline, phenol, and water.
Nitroxide Spin Labels for Exploring Relationships Between …
2024年9月14日 · Cw EPR at high magnetic fields allows one to resolve the nitroxide g-tensor principal components. The g xx component is particularly sensitive to the microenvironment. In addition, high-field ENDOR or ELDOR-detected NMR (EDNMR) can favorably be used to measure the nitrogen hyperfine-tensor and quadrupole-tensor …
Guidelines for the Simulations of Nitroxide X-Band cw EPR
2023年1月31日 · Here, we consider SDSL-EPR based on the selective grafting of a nitroxide on the protein under study, followed by X-band cw EPR analysis. To extract valuable quantitative information from SDSL-EPR spectra and thus give a reliable interpretation on biological system dynamics, a numerical simulation of the spectra is required.
Exploring Structure, Dynamics, and Topology of Nitroxide Spin …
2015年1月1日 · This chapter introduces methods commonly employed for SDSL and describes selected CW EPR-based methods that can be applied to (1) map secondary and tertiary protein structure, (2) determine membrane protein topology, (3) measure protein backbone flexibility, and (4) reveal the existence of conformational exchange at equilibrium.
Guidelines for the Simulations of Nitroxide X-Band cw EPR
2023年1月31日 · Site-directed spin labeling (SDSL) combined with continuous wave electron paramagnetic resonance (cw EPR) spectroscopy is a powerful technique to reveal, at the local level, the dynamics of structural transitions in proteins. Here, we consider SDSL-EPR based on the selective grafting of a nitroxide …
Analyzing CW EPR Spectra of Nitroxide Labeled …
Programs to simulate and fit CW EPR spectra have been around for a long time. Except for a very well-defined model system, an experimental spectrum of a spin labeled protein is typically a mix of multiple states. This article focuses on the analysis of the CW spectrum in several stages of detail, from qualitative to detailed.
Guidelines for the Simulations of Nitroxide X-Band cw EPR …
2023年1月31日 · Site-directed spin labeling (SDSL) combined with continuous wave electron paramagnetic resonance (cw EPR) spectroscopy is a powerful technique to reveal, at the local level, the dynamics of...
EPR Spectroscopy of Nitroxide Spin Probes | Request PDF
2017年6月19日 · The nitroxide continuous wave (CW) EPR spectrum is particularly sensitive to its environment, and it gives information on backbone and side chain fluctuation at the labeling site at a time scale...
- 某些结果已被删除