How do you calculate serial dilutions? + Example - Socratic
2014年6月18日 · You multiply the original concentration by the dilution factors for each dilution. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step. In serial dilutions, you multiply the dilution factors for each step. The dilution factor or the dilution is the initial volume divided by the final volume. DF = …
How do you perform a serial dilution? + Example - Socratic
2017年2月4日 · You do it by steps for better accuracy. Example: If I wanted a dilution of 1:100, I could measure out 1 mL and dilute it to 100 mL in one go. With the 1mL pipette I have the accuracy would be +-0.05mL at best, which is 5% If I first measure out 10mL +-0.05=0.5% and dilute it to 100mL+-0.15=0.15% in a measuring flask, and then take 10mL+-0.05=0.5% out of this flask to another one of 100mL+-0.15 ...
Dilution Calculations - Chemistry - Socratic
In a serial dilution, 0.1 mL of serum is added to 1.9 mL of saline in the first tube. The next tubes contain 1.5 mL of saline, and 0.5 mL from the previous tube is added to the next tube. How do we calculate dilution in the next tubes?
In a serial dilution, 0.1 mL of serum is added to 1.9 mL of saline in ...
2015年12月19日 · Here's how you do it. > You can see the general method of calculating dilution factors in this Socratic answer. The general method for calculating serial dilutions is in this answer The general formula is for calculating a single dilution factor is "DF" = V_f/V_i Here you have two dilution factors, one for the first dilution and another for the other dilutions. First dilution : …
In a serial dilution, if tube 1 is undiluted and twofolds ... - Socratic
2018年6月5日 · Well, you are successively diluting a stock solution, of (apparently) known concentration. But you really have not explained your experiment. As always, "concentration"="Moles OR mass of stuff"/"Volume of solution"...and typically we have units of mol*L^-1... We need more detail with respect to the experiment.
How do we dilute a bacterial culture 50-fold, 100-fold, and
2015年7月15日 · You could do the dilutions in one step, or you might have to do serial dilutions. > There is a limit to how much you can dilute a sample in one step. You probably wouldn't want to dilute by more than a factor of 100 in a single step, because that would give you a large volume of dilute solution. Rather, you would use the serial dilution technique. Remember the formula for dilution factor ("DF ...
Question #269be + Example - Socratic
2015年2月5日 · When you're doing a serial dilution, each tube will dilute the sample by the same calculated dilution factor. Notice how in the above example each solution will be diluted by a factor of 10. If you apply this method to your dilution, you'll get a dilution factor for tube 4 equal to . #DF_("final tube") = "1:20"^(4) = "1:160,000"#
How can I calculate dilution concentration? + Example - Socratic
2014年8月10日 · You use the formula c_1V_1 = c_2V_2. See How do you do dilution calculations? EXAMPLE Describe how would prepare 50 mL of a 1.0 mol/L solution from a 2.0 mol/L stock solution. Solution Your first step is to calculate the …
How do you calculate dilution factor? + Example - Socratic
2014年3月22日 · The dilution factor is often used as the denominator of a fraction. For example, a "DF" of 100 means a 1:100 dilution. EXAMPLE 2: How would you make 500 mL of a 1:250 dilution?
How does dilution affect the pH of a basic solution? - Socratic
2014年5月5日 · Dilution reduces the pH of a basic solution. > The pH of a basic solution is greater than 7. As you dilute a solution, it becomes more and more like pure water. So the pH moves closer to the pH of pure water, pH 7. The pH decreases on dilution. Example: What is the pH of a 10⁻³ mol/L solution of NaOH? What is the pH if you dilute 100 mL of this solution to 1 L? Solution: pOH = -log[OH ...