Single‐cell RNA sequencing technologies and applications: A …
Single‐cell RNA sequencing (scRNA‐seq) technology has become the state‐of‐the‐art approach for unravelling the heterogeneity and complexity of RNA transcripts within individual cells, as well as revealing the composition of different cell types and ...
Single-cell RNA sequencing technologies and bioinformatics …
2018年8月7日 · Single-cell RNA sequencing (scRNA-seq), for example, can reveal complex and rare cell populations, uncover regulatory relationships between genes, and track the trajectories of distinct cell...
A practical guide to single-cell RNA-sequencing for biomedical …
2017年8月18日 · In this review, we present a practical guide to help researchers design their first scRNA-seq studies, including introductory information on experimental hardware, protocol choice, quality control, data analysis and biological interpretation.
Single-Cell RNA Sequencing Procedures and Data Analysis
2021年3月20日 · A typical single-cell sequencing workflow involves initial tissue and cell preparation, cell capturing and library preparation, sequencing and raw data processing, as well as visualization and downstream analyses.
Single cell RNA-seq: An introductory overview and tools for …
10x Genomics’ single cell RNA-seq (scRNA-seq) technology, the Chromium Single Cell 3’ solution, allows you to analyze transcriptomes on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries.
Introduction to Single-Cell RNA Sequencing - PubMed
By isolating single cells, capturing their transcripts, and generating sequencing libraries in which the transcripts are mapped to individual cells, scRNA-seq allows assessment of fundamental biological properties of cell populations and biological systems at unprecedented resolution.
The Evolution of Single-Cell RNA Sequencing Technology and …
As an emerging sequencing technology, single-cell RNA sequencing (scRNA-Seq) has become a powerful tool for describing cell subpopulation classification and cell heterogeneity by achieving high-throughput and multidimensional analysis of individual ...
The Technology and Biology of Single-Cell RNA Sequencing - Cell …
2015年5月21日 · Recently developed single-cell mRNA-sequencing methods enable unbiased, high-throughput, and high-resolution transcriptomic analysis of individual cells. This provides an additional dimension to transcriptomic information relative to traditional methods that profile bulk populations of cells.
Applications of single-cell RNA sequencing in drug discovery …
2023年4月28日 · Single-cell technologies, particularly single-cell RNA sequencing (scRNA-seq) methods, together with associated computational tools and the growing availability of public data resources, are...
Single-Cell RNA Sequencing Analysis: A Step-by-Step Overview
Since its introduction, single-cell RNA sequencing (scRNA-seq) approaches have revolutionized the genomics field as they created unprecedented opportunities for resolving cell heterogeneity by exploring gene expression profiles at a single-cell resolution.
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