YAC-1 - TIB-160 - ATCC
YAC-1 is a lymphoma which was induced by inoculation of the Moloney leukemia virus (MLV) into a newborn A/Sn mouse. The cells are sensitive to the action of natural killer (NK) cells and are useful in assays of NK cell activity.
Cell death and cytotoxic effects in YAC-1 lymphoma cells …
2007年2月28日 · YOPRO-1 (YP) and propidium iodide (PI) dye uptake indicated all forms of Hg tested were toxic at concentrations ranging from 25.8-48.4 microM, with two distinct patterns of effects. Early apoptosis was prolonged for CH3HgCl- and TMS-treated cells, with more than 50% remaining YP+/PI- after 4h.
Immunosuppression by YAC-1 lymphoma: role of shed gangliosides
1996年10月10日 · The immunosuppressive activity of YAC-1 supernatant was evident early in the IL-2 signaling pathway, and it had no permanent effect on proliferative capacity of the cells. Inhibition of DNA synthesis was reversed by high levels of IL-2, and YAC-1 supernatants blocked binding of [125I]IL-2 to high-affinity IL-2 receptors.
Separation and identification of GM1b pathway Neu5Ac- and
Monosialoganglioside fraction of YAC-1 lymphoma cells was comprehensively analyzed and structurally defined by nano-high-performance liquid chromatography (nanoHPLC) in on-line conjunction with electrospray ionization quadrupole time-of …
Selective natural killer resistance in a clone of YAC lymphoma cells.
YAC lymphoma cells were treated with the mutagen N-methyl-N'-nitro-N-nitrosoguanidine and then cloned and subcloned. Of 51 clones, 3 were selected for further study. Ten-fold more natural killer (NK) effector cells were required to lyse YAC clone 6 and subclone 6-28 cells compared with clone 19 cells or the YAC parent cell line.
Genetic studies on natural resistance to Moloney lymphoma (YAC ...
YAC is a Moloney-virus-induced lymphoma of strain A/Sn origin that is highly sensitive to natural killing (NK) in vitro and NK-mediated hybrid resistance in vivo. Previous studies have shown that hybrid resistance is under polygenic control that includes both H-2 -linked and non- H-2 …
[PDF] Shedding and immunoregulatory activity of YAC-1 lymphoma …
Sialoglycoconjugates and glycosphingolipids were quantitated in a series of variants derived from the YAC‐1 lymphoma, known to be highly sensitive to natural killer (NK)‐cell‐mediated lysis. The …
Shedding and Immunoregulatory Activity of YAC-1 Lymphoma …
1983年8月1日 · YAC-1 lymphoma cells, both when cultured in vitro and when passaged in ascites form in vivo, synthesize gangliosides (means of 22.1 and 14.7 nmol lipid-bound sialic acid isolated per 10 8 cells, respectively) with potent inhibitory effects on mitogen- and antigen-induced lymphoproliferation: 10 to 30 nmol highly purified YAC-1 gangliosides/ml ...
Cell death and cytotoxic effects in YAC-1 lymphoma cells …
2007年2月28日 · The effects of 1 min–4 h exposures to four Hg compounds (mercuric chloride [HgCl 2], methyl mercuric chloride [CH 3 HgCl], p-chloromercuribenzoate [p-CMB] and thimerosal [TMS; ethylmercurithiosalicylate]) on cell death, microtubules, actin, CD3 receptor expression, protein tyrosine phosphorylation (PTyr-P) and intracellular calcium ([Ca 2+] i ...
Polyunsaturated fatty acids induce cell death in YAC-1 lymphoma …
On the basis of these results, we can speculate that long-chain polyunsaturated fatty acids such as EPA and AA as well as LNA induce cell death in YAC-1 lymphoma by an independent mechanism of caspase-3 activation.