Fluorescence spectroscopy - Wikipedia
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
Fluorimetry: Principle, Instrumentation, Factors, Uses - Microbe …
2024年4月27日 · Fluorimetry is a scientific and analytical technique used to detect and measure the fluorescent light emitted by the sample (lying in the visible spectrum) with the aid of ultraviolet rays. Here, the wavelength of the emitted light by the substance is greater than the absorbed light.
Fluorimetry | Principle, Instrumentation and Applications
2019年3月26日 · Fluorimetry is a type of spectroscopy that measures the emitted radiation from a substance. This radiation is one that is emitted by the substance when the electrons transit from the excited state to the ground state. In UV-Visible spectroscopy, the excitation wavelength is measured. But, here, the emission wavelength is measured.
fluorimetry - 百度百科
fluorimetry，英语单词，主要用作名词，作名词时译为“荧光分析， [分化] 荧光测定法 ”。 [1] In this paper, the progress of the fluorimetry in pharmaceutical analysis in recent years is reviewed. 论述了近几年荧光分析法在药物分析中的研究进展。 The common analytical methods include spectrophotometry and fluorimetry, but they have some serious disadvantages. 常用的方法主要是 分光光度法 和荧光法，但它们都存在严重的缺陷。
Fluorometer - Wikipedia
Modern fluorometers are capable of detecting fluorescent molecule concentrations as low as 1 part per trillion. Fluorescence analysis can be orders of magnitude more sensitive than other techniques. Applications include chemistry / biochemistry, medicine, environmental monitoring.
Fluorometry - an overview | ScienceDirect Topics
Fluorometry is superior to spectrophotometry in terms of sensitivity and specificity. In general, the sensitivity of fluorescence is 10–1000-fold higher in comparison to absorbance measurements. Fluorometry was introduced in immunological assays to improve immunoassay sensitivity.
Fluorescence Spectrophotometer: Principle, Components, …
2024年1月20日 · A fluorescence spectrophotometer, often known as fluorimetry or spectrofluorometry, is a sophisticated branch of electromagnetic spectroscopy that analyzes the fluorescence generated by a specimen. This approach uses a specialized laser beam, primarily in the UV range, to activate the electrons in certain chemical compounds.
When the external energy supply is by means of the absorption of infrared, visible or ultraviolet light, the emitted light is called photoluminescence and this is the process that takes place in any fluorimetric analysis.
Fluorimetry-Principle and Applications - Notes for Biology
Fluorimetry is a potent and adaptable analytical technique that uses molecules’ fluorescence characteristics to detect and quantify a wide range of chemicals. Its great sensitivity and specificity make it useful in a variety of applications, including basic biological research, clinical diagnostics, environmental monitoring, and industrial ...
Principles and Theory of Fluorescence Spectroscopy - HORIBA
Fluorescence spectroscopy analyzes fluorescence from a molecule based on its fluorescent properties. Fluorescence is a type of luminescence caused by photons exciting a molecule, raising it to an electronic excited state.