pET-28a(+) Sequence and Map - SnapGene
Bacterial vector for expression of N-terminally 6xHis-tagged proteins with a thrombin site. For other reading frames, use pET-28b (+) or pET-28c (+). Sequence Author: MilliporeSigma …
pET-28a (+) vector map and sequence
pET-28a (+) is a bacterial expression vector designed for the production of N-terminally 6xHis-tagged proteins with a thrombin cleavage site. This vector is particularly suitable for proteins that require post-translational processing to remove the His-tag. 1. Centrifuge at 5,000×g for 5 min. 2.
Addgene: Vector Database - pET-28 a (+)
There is a problem with the plasmid I received. What strain of bacteria does my stab contain? How can I be notified when a plasmid from a specific lab or paper is available? What is virus associated DNA, and why do I have to order it? Welcome to Vector Database!
69864 Sigma-Aldrich pET-28a(+) DNA - Novagen - EMD Millipore
pET-28a (+) DNA - Novagen The Novagen pET-28a-c (+) vectors carry an N-terminal His•Tag/thrombin/T7•Tag configuration plus an optional C-terminal His•Tag sequence. - Find MSDS or SDS, a COA, data sheets and more information.
pET-28a(+) - EcoliWiki
2011年8月16日 · The pET-28a (+) vector carries an N-terminal His•Tag/thrombin/T7•Tag configuration in addition to an optional C-terminal His•Tag sequence. Unique sites are shown on the circle map. The T7 expression region is reversed on the circular map due to the sequence numbering from the pBR322 convention.
DNASU Plasmid | Detailed Vector Information: pET28a
bacterial expression, in vitro transcription, multiple cloning site, with tag/fusion/marker. Midwest Center for Structural Genomics.
pET-28a (+) (VET1494) - Creative Biogene
pET28a is the most popular expression plasmid on the market (described in over 40,000 published articles). It contains the T7 promoter and the adjacent lac operator sequence, which acts to repress non-induced expression.
Improved designs for pET expression plasmids increase protein ...
We present improved designs and demonstrate that, when incorporated into pET28a, they support increases in protein production. The improved designs are applicable to most of the 103 vectors in the pET series and can be easily implemented.
pET-28a(+)::NL - Addgene
Plasmid pET-28a (+)::NL from Dr. Andrew Millar's lab contains the insert NLUC and is published in Plant Methods. 2019 Jul 8;15:68. doi: 10.1186/s13007-019-0454-4. eCollection 2019. This plasmid is available through Addgene.
pET-28a(+)-sumo vector map and sequence - novoprolabs.com
Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg. An e.coli expression vector pET-28a fused with N-His, N-Thrombin, and N-sumo. Designed for Ni-NIA affinity purification and tag removal. 1. Centrifuge at 5,000×g for 5 min. 2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA. 3.