A spheroid whole mount drug testing pipeline with machine …
2024年12月18日 · Here, we developed a high-content pipeline ranging from the setup of novel tumor-fibroblast spheroid co-cultures over optical tissue clearing, whole mount staining, and 3D confocal microscopy to optimized 3D-image segmentation and a 3D-deep-learning model to automate the analysis of a range of cell-type-specific processes, such as cell ...
Multispectral confocal 3D imaging of intact healthy and tumor …
2022年9月30日 · We demonstrate effective mLSR-3D imaging of various tissues, including patient-derived organoids and xenografted tumors, and, furthermore, describe an optimized workflow for mLSR-3D imaging...
Deep Learning–Based 3D Single-Cell Imaging Analysis Pipeline …
2024年2月15日 · Here, we developed a 3D live-cell imaging assay using confocal microscopy of patient-derived tumor organoids and a software tool, SiQ-3D (single-cell image quantifier for 3D), that optimizes deep learning (DL)–based 3D image segmentation, single-cell phenotype classification, and tracking to automatically acquire multidimensional dynamic data ...
3D imaging for driving cancer discovery - PMC - PubMed Central …
Confocal and two‐photon microscopy resolves refined tumour growth patterns. As opposed to light sheet technology, confocal or two‐photon microscopes offer more mainstream systems for large‐scale 3D imaging with higher resolution, yet at the sacrifice of some working depth (Box 1).
Types of spectroscopy and microscopy techniques for cancer …
A confocal Raman microscopy was used for lung cancer detection. The stained sections of normal lung tissue were used as a reference and scanned with a microscope. Data reduction by principal component analysis (PCA) was used in combination with Raman microscopy to analyze the variation between the spectra and group them using similarities.
Noninvasive, label-free, three-dimensional imaging of melanoma …
2016年7月22日 · Here, we developed a confocal photothermal microscope for noninvasive, label-free, three-dimensional imaging of melanoma. The axial resolution of confocal photothermal microscope is ~3...
Spectral mapping of 3D multi-cellular tumor spheroids: time-resolved ...
2016年6月7日 · A tumor-like multi-cellular spheroid (3D) differs from a 2D cell in a number of ways. This is demonstrated using time resolved confocal microscopy. Two different tumor spheroids – HeLa (cervical cancer) and A549 (lung cancer) – are studied using 3 different fluorescent dyes – C153 (non-covalent), CPM (covale
A high-precision multi-dimensional microspectroscopic technique …
2023年5月29日 · The divided-aperture differential 3D confocal Geometry-Raman-Brillouin microscopy (DDCGRBM) experiments on gastric cancer tissues and adjacent normal tissues showed that the concentration...
3D microscopy gives more accurate cancer diagnosis
2017年10月3日 · A novel microscopy technique to examine tumour tissue in three dimensions can more accurately diagnose cancer than current two-dimensional methods, according to a study conducted at Karolinska...
Acoustic virtual 3D scaffold for direct-interacting tumor organoid ...
2024年11月22日 · In this study, we developed an acoustic virtual 3D scaffold (AV-Scaf) method to achieve 3D tumor organoid culture, which enabled a direct-interacting tumor organoid–immune cell coculture system. Specifically, AV-Scaf involves a designed vortex acoustic field generated by a 20-MHz US transducer and a lens, achieving contact-free clustering and ...