Cell-free gene expression | Nature Reviews Methods Primers
2021年7月15日 · Cell-free gene expression (CFE) emerged as an alternative approach to living cells for specific applications in protein synthesis and labelling for structural biology and proteomics...
Cell-Free Protein Expression | NEB
Cell-free protein synthesis (CFPS) is a protein expression approach that enables production of a target protein without the use of living cells. In CFPS, a solution containing all the cellular machinery needed to direct protein synthesis (e.g., ribosomes, tRNAs, enzymes, cofactors, amino acids, etc.) is used to transcribe and translate a ...
A User’s Guide to Cell-Free Protein Synthesis - MDPI
2024年2月1日 · Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more.
Cell-Free Protein Expression | Thermo Fisher Scientific - US
In vitro protein expression (also known as in vitro translation, cell-free protein expression, cell-free translation, or cell-free protein synthesis) is a technique that enables researchers to rapidly express and manufacture small amounts of functional proteins.
Cell-Free Protein Synthesis: Applications Come of Age
Cell-free protein synthesis systems exploit crude cell extracts to produce valuable therapeutics and vaccines, among other products. The driving force behind the development of this technology has been its potential to rapidly express bioactive recombinant DNA (rDNA) proteins.
Cell-Free Protein Synthesis - an overview | ScienceDirect Topics
Cell-free protein synthesis employs cellular extracts to direct protein synthesis from added genetic material, offering a number of advantages over cell-based systems in the production of proteins.
Cell-Free Protein Synthesis: Methods and Protocols - Springer
Cell-Free Protein Synthesis: Methods and Protocols brings together the key opinion leaders of cell-free technology development and provides case studies and detailed protocols for the application of cell-free methodology.
Engine out of the Chassis: Cell-Free Protein Synthesis and its Uses
2014年1月1日 · Cell-free protein synthesis and its functionalities. Translation commences upon the addition of DNA (PCR product or plasmid) in a coupled system or by adding separately transcribed mRNA. Modified CFPS may exhibit various functionalities, some of …
Cell-Free Protein Synthesis: Chassis toward the Minimal Cell
2019年4月5日 · The cell-free protein synthesis (CFPS) techniques harness the central cellular activity of transcription/translation in an open environment, providing the framework for multiple cellular processes assembling.
A User's Guide to Cell-Free Protein Synthesis - PubMed
2019年3月12日 · Cell-free protein synthesis (CFPS) is a platform technology that provides new opportunities for protein expression, metabolic engineering, therapeutic development, education, and more. The advantages of CFPS over in vivo protein expression include its open system, the elimination of reliance on livi …
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