DAPI Protocol for Fluorescence Imaging - Thermo Fisher Scientific
Step-by-step protocol for the use of DAPI (4′,6-diamidino-2-phenylindole) for nuclear acid (nuclear) staining in fluorescence microscopy.
DAPI Counterstaining Protocols | Thermo Fisher Scientific - US
DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other structures. When used according to our protocols, DAPI stains nuclei specifically, with little or no cytoplasmic labeling.
DAPI Staining Protocol - McGill University
DAPI Staining Protocol Prepare the following solutions: (To determine the amount needed for your experiment, please note that one tube of Solution 1 will yield a total of 100mL of working solution, at a final concentration of 0.5ug/mL.)
Hoechst & DAPI Staining Protocols – Cell Staining with
Performing nuclear staining with DAPI or Hoechst? Check out Biotium's detailed Hoechst & DAPI staining protocols for staining live cells or fixed cells & tissues.
Labeling Nuclear DNA Using DAPI - CSH Protocols
A number of fluorescent stains are available that label DNA and allow easy visualization of the nucleus in interphase cells and chromosomes in mitotic cells, including Hoechst, 4′,6-diamidino-2-phenylindole (DAPI), ethidium bromide, propidium iodide, and acridine orange.
DAPI is a popular nuclear counterstain for use in multicolor fluorescent techniques. Its blue fluorescence stands out in vivid contrast to green, yellow, or red fluorescent probes of other structures. When used according to our protocols, DAPI stains nuclei specifically, with little or no cytoplasmic labeling.
DAPI Staining Protocol Prepare the following solutions: (To determine the amount needed for your experiment, please note that one tube of Solution 1 will yield a total of 100mL of working solution, at a final concentration of 0.5ug/mL.) Solution 1*: 5 μL DAPI (10 mg/ml solution) 995 μL PBS
4',6‐diamidino‐2‐phenylindole (DAPI) is a fluorescent DNA stain useful for multiple purposes including, cell cycle studies, determining the mitotic index of an organism or enumerating unicellular protists and bacteria.
Cells that have been immunolabeled can be stained with DAPI by starting at Step 7. 1. Dilute the DAPI stock solution 1:5000 in PBS +. 2. Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS +. Do not allow the cells to dry out at any time during the protocol. 3. Fix the cells for 10 min in 3.7% ...
Cell assay, fix and stain (DAPI, Phalloidin)
This is the DRSC's basic protocol for fixation with formaldehyde and staining with DAPI and/or phalloidin. It is also appropriate for fixation prior to immunostaining, although some antibodies, cell types, target proteins, etc. might require a modified protocol.
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