An Introduction to Fluorescence | Science Lab - Leica Microsystems
2023年6月2日 · For microscopy, fluorescence is the most useful kind of luminescence. Fluorochromes can easily be excited with their specific wavelength via certain light sources, e.g., lamps and filter systems or lasers, and the emitted light can be distinguished from the excitation light by the wavelength (Stokes’ shift).
What is FLIM - Fluorescence Lifetime Imaging Microscopy?
2022年10月17日 · FLIM exploits fluorescence lifetime which is a measure of how long a fluorescent molecule or fluorophore remains on average in its excited state before returning to the ground state by emitting a fluorescence photon. The emission of a fluorescence photon from a fluorophore does not always occur at an exact time after excitation. Instead, a distribution of …
The Fundamentals and History of Fluorescence and Quantum Dots
2017年11月15日 · At some point in your research and science career, you will no doubt come across fluorescence microscopy. This ubiquitous technique has transformed the way in which microscopists can image, tag and trace anything from whole organisms to single proteins and beyond. In this article, we will examine what is meant by "fluorescence", the history and basic …
Fluorescence | Applications - Leica Microsystems
Fluorescence is a form of luminescence that through microscopy allows users to determine the distribution of a single molecule species, its amount and its localization inside a cell. Colocalization and interaction studies can be performed, and ion concentrations as well as intra- and intercellular processes like endocytosis and exocytosis can ...
Fluorescence in Microscopy | Science Lab | Leica Microsystems
2011年4月27日 · Fluorescence microscopy is widely used and offers great specificity. Various techniques make it possible to address different problems and even to circumvent the diffraction limit that was described by Ernst Abbe. The localization of a molecule species can be determined with a co-staining of organelles, e.g. the cytoskeleton or membranes.
How to Prepare your Specimen for Immunofluorescence Microscopy
2022年1月10日 · Immunofluorescence (IF) is a powerful method for visualizing intracellular processes, conditions and structures. IF preparations can be analyzed by various microscopy techniques (e.g. CLSM, Epifluorescence, TIRF, GSDIM), depending on the application or the researcher’s interest. Meanwhile, IF has become indispensable for a large number of research …
Epi-Illumination Fluorescence and Reflection-Contrast Microscopy ...
2023年11月2日 · Compared to today, Fluorescence microscopy was applied for the first time with the use of transmitted light and darkfield microscopy, due to its limited range of applications at that time. But with its increasing importance for histology, cytology, molecular biology, and immuno-diagnosis, the demand for a fundamental improvement of illumination ...
Total Internal Reflection Fluorescence (TIRF) Microscopy
2015年3月2日 · Total internal reflection fluorescence (TIRF) is a special technique in fluorescence microscopy developed by Daniel Axelrod at the University of Michigan, Ann Arbor in the early 1980s. TIRF microscopy delivers images with an outstandingly high axial resolution below 100 nm. This allows the observation of membrane-associated processes.
Studying Virus Replication with Fluorescence Microscopy
2023年11月15日 · Amongst sequencing techniques, bioinformatics, and electron microscopy, Ogando et al. analyzed infected cells by fluorescence microscopy: Vero E6 cells were grown on glass cover slips, infected with SARS-CoV-2, and fixed with paraformaldehyde. Then, the cells were incubated with antisera from rabbits or mice which were exposed to SARS-CoV ...
Leica M205 FCA & Leica M205 FA Fluorescence stereo microscopes
The Leica M205 FA opens up a new world of research in fluorescence microscopy, for instance when working in a sterile cabinet. Easily handle complex multi-channel fluorescence imaging procedures Execute demanding experiments with the motorized zoom, filter changer, Fluroescence Intensity Manager (FIM), and iris diaphragm