Gene transfer to plants by electroporation: methods and applications ...
Analysis with discriminative molecular markers demonstrated that two artificial gene transfer methods such as particle bombardment and intact cell electroporation are better in production of transgenic rice (O. sativa L.) plants (cv. Lido, Carnaroli …
Gene transfer to plants by electroporation: methods and applications
2020年4月1日 · Electroporation is an electrical transformation method by which transient electropores are produced in the cell membranes. Based on the applications, process can be either reversible where...
Electroporation Method Of Gene Transfer - Bio Smart Notes
2024年10月12日 · The electroporation method of gene transfer is based on the use of electrical impulses of high field strength. These impulses increase the permeability of the protoplast membrane and facilitate the entry of DNA molecules into the cells if the DNA is in direct contact with the membrane.
Transfection by Electroporation - PMC - PubMed Central (PMC)
2021年11月8日 · Electroporation—the use of high-voltage electric shocks to introduce DNA into cells—is a procedure that is gaining in popularity for standard gene transfer and also allows the generation of genetically modified mice,.
Gene transfer to plants by electroporation: methods and ... - PubMed
Developing gene transfer technologies enables the genetic manipulation of the living organisms more efficiently. The methods used for gene transfer fall into two main categories; natural and artificial transformation. The natural methods include …
Plant Gene Transfer Using Electrofusion and Electroporation
In recent years, the rapidly developing fields of electrofusion of somatic cells and electroporation of selected cell types have become an extremely important tool in the production and modification of hybrid somatic gene pools. In plants, these procedures have thus...
Successful Gene Transfer in Plants Using Electroporation and ...
Electroporation and electrofusion of plant tissues are rapid, reliable, techniques for genome modification. To accomplish these procedures two different wave forms have been developed and successfully used in plants during the last decade. We have shown that both...
Gene transfer to plants by electroporation: methods and …
Overall results showed that electroporation could be used to deliver specific genes into oil palm calli and might be developed further to increase its efficiency. Advancements in plant transformation: from traditional methods to cutting-edge techniques and emerging model species.
Gene transfer to plants by electroporation: methods and …
2020年4月2日 · Electroporation is an electrical transformation method by which transient electropores are produced in the cell membranes. Based on the applications, process can be either reversible where electropores in membrane are resealable and cells preserve the vitality or irreversible where membrane is not able to reseal, and cell eventually dies.
Plant Gene Transfer Using Electrofusion and Electroporation
We propose a model of generating triploid plants by somatic cell fusion to transfer limited genomic information from an alien plant to a crop plant. Somatic hybridization has some novel features but, in practice and conception, it is an extension of the methods of sexual hybridization.