Enzymology and significance of protein histidine methylation
Protein histidine methylation (Hme) was an elusive PTM for over 5 decades and has only recently attracted considerable attention through discoveries concerning its enzymology, extent, and function. Here, we review the status of the Hme field and discuss the implications of Hme in physiological and cellular processes.
Protein Histidine Methylation - PubMed
However, the molecular identification of the first two distinct histidine-specific protein methyltransferases has been established in yeast (Hpm1) and in metazoan species (actin-histidine N-methyltransferase), giving new insights into the phenomenon of …
Molecular basis for METTL9-mediated N1-histidine methylation
2023年4月4日 · Histidine methylation occurs at the N1 or N3 position of the imidazole ring and accounts for ~13% of protein methylation events 3.
Large-scale identification of protein histidine methylation in …
2021年5月22日 · There are currently no methods to enrich histidine methylated peptides for mass spectrometry analysis and large-scale studies of the modification are hitherto absent. Here, we query ultra-comprehensive human proteome datasets to generate a resource of histidine methylation sites.
Molecular basis for histidine N1 position-specific methylation by ...
2018年2月20日 · Besides arginine and lysine methylations, histidine can also undergo methylation in proteins or histidyl peptides, such as ribosome protein Rpl3, actin, 1, 2 and dipeptide carnosine...
Molecular basis for protein histidine N1-specific methylation of …
Histidine methylation serves as an intriguing strategy to introduce altered traits of target proteins, including metal ion chelation, histidine-based catalysis, molecular assembly, and translation regulation.
Histidine Nτ-methylation identified as a new posttranslational ...
To determine whether histidine residue (s) of histone proteins are methylated, and if so, which isomer of methylhistidine is introduced, we adopted an approach that allows to distinguish between N τ and N π histidine methylation by detecting methylhistidine isomers after acid hydrolysis of the protein of interest ( Fig. 1B ).
Protein histidine methylation (Hme) was an elusive PTM for over 5 decades and has only recently attracted considerable attention through discoveries concerning its enzymology, extent, and function. Here, we review the status of the Hme eld and discuss the implications of Hme in physio- logical and cellular processes.
The methyltransferase METTL9 mediates pervasive 1 ... - Nature
2021年2月9日 · Protein histidine methylation, occurring as the two isomers 1- and 3-methylhistidine (1MH and 3MH), was first reported five decades ago, but remains largely unexplored. Here we report that METTL9...
Large-scale identification of protein histidine methylation in …
2021年5月22日 · Here, we query ultra-comprehensive human proteome datasets to generate a resource of histidine methylation sites. In HeLa cells alone, we report 299 histidine methylation sites as well as 895 lysine methylation events.