DAPI - Wikipedia
DAPI (pronounced 'DAPPY', /ˈdæpiː/), or 4′,6-diamidino-2-phenylindole, is a fluorescent stain that binds strongly to adenine–thymine-rich regions in DNA. It is used extensively in fluorescence microscopy .
DAPI: a DNA-specific fluorescent probe - PubMed
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids.
DAPI and antibody staining techniques. The combination of DAPI and a fluorescent labeled antibody is a powerful cytochemical tool for concurrent visualization of DNA and individual proteins in the cell. Using this technique, DNA and tubulin, microtubules or microfilaments can be identified (Baumstark Khan et al. 1984, Roos et al. 1984. Wang et ...
DAPI - an overview | ScienceDirect Topics
2012年2月7日 · In DAPI-treated mammalian cells, some studies revealed an unexpectedly homogeneous and/or diffuse distribution of mtDNA [56,57], while others depicted discrete punctate structures scattered throughout mitochondria [53,58,59].
DNA-DAPI Interaction-Based Method for Cell Proliferation Rate ...
Here, we report a novel technique for the DNA content-based evaluation of cell proliferation using DNA-binding dye DAPI. We demonstrated the method’s compatibility with four different cell cultures: cancer lines MCF-7 and MH-22a, embryonic fibroblast cell line Swiss 3T3, and primary mesenchymal stem cell culture isolated from rat’s incisors.
Single-cell analysis of bidirectional reprogramming between early ...
2 天之前 · 2 Biochemistry, Cell and Molecular Biology Program, Weill Cornell Graduate School of Medical Sciences, New York, NY 10021, USA. ... DAPI and BV421 were excited at 405 nm and detected using 450/50 nm band-pass filters. GFP and Venus were excited at 488 nm and detected using 525/50 nm band-pass filter. mCherry was excited at 561 nm and detected ...
Cell-cycle-dependent mRNA localization in P-bodies - Cell Press
2024年11月7日 · Nuclei were segmented using the DAPI channel, and the cytoplasm using the DDX6 IF channel using an intensity threshold of 350-450. To determine the cell cycle phase based on the nuclear FUCCI signal, we used the Otsu method to classify nuclei as positive or negative in the green and red channels.
DAPI Dyes - Probes / BOC Sciences
DAPI (pronounced "DAPPY"), or 4 ', 6-diamidino-2-phenylindole, is a fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is widely used in fluorescence microscopy. Because DAPI can penetrate intact cell membranes, it can be used to stain living and fixed cells, although DAPI is less efficient at crossing membranes in ...
AptaFluorescence: An aptamer-based fluorescent imaging …
1 天前 · The nuclei were then stained with DAPI for 10 minutes, followed by two washes with TBST. Then, the silicone sealing gasket was removed, and the mounting media and coverslips were applied. The glass slide was cured at 4°C overnight before imaging with a Zeiss live cell observer at 20x, 40x and 63x magnification levels.
Modularity-based mathematical modeling of ligand inter …
5 天之前 · b Computation of the DAPI-positive cell density (n = 6–9 biological replicates; ... to assess molecular signaling that governs # ligand inter-cluster edges-mediated regulation of stem cell ...