Automatic stage identification of Drosophila egg chamber …
2016年1月6日 · Egg chamber areas of DAPI images proved to be a stable feature for stage differentiation, while oocyte size played a critical role for late stage differentiation.
Dynamic Cytoophidia during Late-Stage Drosophila Oogenesis
2024年1月7日 · We culture the late-stage egg chamber of CTPS-mCherry transgenic flies in vitro and observe the morphology of the egg chambers and the dynamics of cytoophidia using live imaging, providing us with the opportunity to capture the details of the changes in cytoophidia.
Shining light on Drosophila oogenesis: live imaging of egg …
Live imaging of RNA movement in the fly ovary was first achieved by microinjection of fluorescently labeled molecules into late stage egg chambers. An active, long-range, microtubule-dependent cytoplasmic flow termed ooplasmic streaming occurs between late …
Egg chamber diagrams from selected stages of Drosophila
Stages 7-10A (the period of slow streaming) represent mid-oogenesis, and stages 10B-14 (including the period of fast streaming) represent late oogenesis. Stage 9: The border cell cluster...
2024年1月6日 · analysis of the cytoophidia in the late stage egg chambers of Drosophila lays the foundation for future understanding of the role of cytoophidia in germline cell development.
Identification of 14 stages of Drosophila egg chamber.
Egg chamber areas of DAPI images proved to be a stable feature for stage differentiation, while oocyte size played a critical role for late stage differentiation.
Live Imaging of Nurse Cell Behavior in Late Stages of
2023年1月31日 · Here, we present a relatively simple protocol for long-term (~2–4 h) live imaging of late-stage egg chambers (stages 10–14), originally developed for capturing the dynamics of cytoplasmic flow during nurse cell dumping in stage 11/12 chambers but readily adaptable to younger egg chambers.
Preparing Individual Drosophila Egg Chambers for Live Imaging
2012年2月27日 · Avoid puncturing any egg chambers by inserting the probe between late stage egg chambers. Pull the dissecting needle through the ovary until it exits at the early stage egg chambers (Figure 4B). When done correctly, the probe will remove the connective tissue holding the ovarioles and late stage egg chambers in place.
A Low-Tech Flow Chamber for Live Imaging of Drosophila Egg
2023年1月31日 · In this chapter, we describe how to dissect and prepare ovariole strands containing stage 1–8 egg chambers and immobilize them in a flow chamber suitable for repeated media exchanges. We have previously published a chapter in Methods in Molecular Biology on live imaging of follicle cell migration [20].
Live imaging of nurse cell behavior in late stages of Drosophila ...
Here, we present a relatively simple protocol for long-term (~2–4 hours) live imaging of late-stage egg chambers (stages 10–14), originally developed for capturing the dynamics of cytoplasmic flow during nurse cell dumping in stage 11/12 chambers but readily adaptable to …