Lipofectamine™ 3000 Transfection Reagent - Thermo Fisher Scientific
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.
Lipofectamine 3000 Transfection Reagent - Thermo Fisher Scientific …
Lipofectamine 3000 reagent outperforms Invitrogen Lipofectamine 2000 and FuGENE HD reagents. Each reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection.
Lipofectamine ™ Reagents Go online to view related products. ∤. Lipofectamine ™ 3000 Reagent is a proprietary formulation for transfecting nucleic acids into a wide range of eukaryotic cells and especially designed for hard to transfect cells. Important Guidelines. ∤. Make DNA-Lipofectamine ™ 3000 complexes in serum-free medium such ...
InvitrogenTM LipofectamineTM 3000 Transfection Reagent was developed to unleash the power of stem cells by providing a highly efficient, cost-effective nucleic acid delivery alternative to electroporation.
Lipofectamine 3000 | Science - AAAS
Lipofectamine 3000 is the company's most efficient and versatile transfection reagent for nucleic acid delivery in the broadest spectrum of cell lines. The product increases efficiency up to 10-fold in difficult-to-transfect cells, which is key for researchers …
Invitrogen™ Lipofectamine™ 3000 Transfection Reagent - Fisher Sci
Lipofectamine™ 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.
Biochemical Invitrogen™ Lipofectamine® 3000 - McKesson
Lipofectamine™ 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types Cell Type: Established Cell Lines, Stem Cells, Primary Cells, Hard-to-Transfect Cells
Lipofectamine 3000 reagent is a versatile reagent that can also be used to deliver siRNA using the same transfection protocol. Simply substitute siRNA for DNA. For this experiment, knockdown of endogenous luciferase was achieved in three engineered luciferase cell lines using Lipofectamine 3000 reagent,
2019年5月5日 · Make DNA-Lipofectamine™ 3000 complexes in serum-free medium such as Opti-MEM™ Reduced Serum Medium and add directly to cells in culture medium, in the presence or absence of serum/antibiotic.
Transfection of (HEK) cells with Lipofectamine 3000 Reagent. Plate cells so they will be 70–90% confluent at the time of transfection. Prepare 2 Eppis for all DNA construct. Eppi 1: Mix 1 volume Opti-MEM with Lipofetamine and vortex. Eppi 2: Mix 1 volume Opti-MEM with P3000 and vortex.