Mapping single-cell-resolution cell phylogeny reveals cell ... - Nature
2021年12月2日 · Here we developed SMALT, a substitution mutation-aided lineage-tracing system that outperforms the available cell barcoding methods in mapping cell phylogeny. We applied SMALT to Drosophila...
专家点评Nature | 胡政/贺雄雷/何真团队合作揭示肿瘤从多克隆至 …
2024年10月31日 · 研究团队利用携带SMALT系统的转基因小鼠，构建了两种小鼠肠癌模型：炎症诱导肠癌模型 （AOM/DSS模型） 和多发性息肉模型 （ApcMin/+模型） 。 基于高分辨率谱系树分析，团队发现大多数 （66.7%） 炎症诱导的早期肠癌和所有Apc息肉均表现出多克隆起源 …
Polyclonal-to-monoclonal transition in colorectal precancerous ... - Nature
2024年10月30日 · Nature - Experiments using DNA barcoding for lineage tracing in mouse models of colorectal cancer reveal polyclonal origins of premalignant lesions, with a transition to monoclonality as they ...
Nature Methods|中山大学贺雄雷:单碱基突变的细胞谱系追踪系统SMALT
2021年12月2日，贺教授团队在Nature 子刊Nature Methods（IF=28）发文“Mapping single-cell-resolution cell phylogeny reveals cell population dynamics during organ development”，介绍一种基于单碱基突变的细胞谱系追踪系统SMALT（substitution mutation aided lineage tracing system），通过SMALT系统构建转基因果蝇品系，首次实现在单细胞水平上对复杂多细胞生物的胚胎发育过程中细胞谱系关系进行可靠描述。 图源论文，点击可查看原文.
Mapping single-cell-resolution cell phylogeny reveals ... - Springer Nature
Here we developed SMALT, a substitution mutation-aided lineage-tracing system that outperforms the available cell barcoding methods in mapping cell phylogeny. We applied SMALT to Drosophila melanogaster and obtained on average more than 20 mutations on a three-kilobase-pair barcoding sequence in early-adult cells.
(PDF) Mapping single-cell-resolution cell phylogeny reveals cell ...
2021年12月1日 · Here we developed SMALT, a substitution mutation-aided lineage-tracing system that outperforms the available cell barcoding methods in mapping cell phylogeny. We applied SMALT to Drosophila...
SMALT-Phylogeny
SMALT (Substitution Mutation-Aided Lineage-Tracing) is a cutting-edge lineage tracing method, utilizing the base editor of engineered AID (activation-induced cytidine deaminase) to induce targeted mutagenesis. This process generates C/G to T/A mutations in a DNA barcode at high efficacy, facilitating the reconstruction of large phylogenetic ...
Mapping single-cell-resolution cell phylogeny reveals cell …
Here we developed SMALT, a substitution mutation-aided lineage-tracing system that outperforms the available cell barcoding methods in mapping cell phylogeny. We applied SMALT to Drosophila melanogaster and obtained on average more than 20 mutations on a three-kilobase-pair barcoding sequence in early-adult cells.
Achieving single-cell-resolution lineage tracing in zebrafish by ...
2024年9月1日 · A recently developed method, a substitution mutation-aided lineage-tracing system (SMALT), successfully reconstructed high-resolution cell phylogenetic trees for Drosophila melanogaster. Here, we implement the SMALT system in zebrafish, recording a median of 14 substitution mutations on a one-kilobase-pair barcoding sequence for one-day post ...
The role of smalt in complex pigment mixtures in Rembrandt’s
2020年9月4日 · To test the effect of smalt on the color of paint mixtures, we compared three different applications of the same smalt paint, each containing a smalt with a different cobalt content: 0 wt% (colorless glass), 2.66 wt% and 4.0 wt% CoO.