Mouse Corneal Epithelial and Stromal Cell Isolation and Culture
Mouse cornea consists of three cellular layers: the epithelium, the stroma, and the endothelium (Figure 1). The epithelium consists of a differentiated superficial squamous cell, a transit wing cell, and a proliferative basal cell type, and is the outmost ocular …
Histological, histochemical, and immunohistochemical …
2024年11月13日 · In the present work, we generated HAC using nanostructured fibrin-agarose biomaterials with cultured corneal epithelial and stromal cells, and we compared the structure and histochemical and immunohistochemical profiles of HAC with control native corneas (CTR-C) and limbus (CTR-L) to determine the level of biomimicry of the HAC with these two na...
Qualitative and Quantitative Evaluation of Donor Corneal Tissue …
We evaluated the corneoscleral discs by slit-lamp microscopy specular microscopy. We analyzed donor corneas quantitatively and qualitatively and graded them accordingly. We also collected blood samples for serological testing and the donor's behavioral and family medical histories.
The new future perspective in corneal tissue utilisation – methods …
2023年6月29日 · We provided morphological (histology, scanning electron microscope) and microbiological analysis in order to compare different methods of corneal lenticule and corneal stromal lamellae preparation and preservation. We also tested the surgical handling of the tissue to secure a safe manipulation of the tissue for clinical use.
Preparation, physico-biochemical characterization, and proteomic ...
2024年10月1日 · In this study, a transparent decellularized cornea was successfully developed using the high hydrostatic pressure method with processing conditions optimized for corneal decellularization.
Cornea Tissue - an overview | ScienceDirect Topics
Cornea tissues are comprised of three layers. Corneal epithelium is the outermost layer which is highly refractive and consists of 4–6 layers of epithelial cells followed by stroma layer constitutes the major part of the cornea and consists of collagen fibers aligned to form a flat lamellar sheet.
Transcriptional Profiling Provides New Insights into Organ Culture ...
Here we demonstrate that MACE provides comparable results for native and FFPE corneal tissue, confirming that the technology is suitable for transcriptome analysis of a wide range of archived diseased corneal samples stored in histological archives.
Unbiased corneal tissue analysis using Gabor-domain optical …
2020年8月7日 · Aim: To improve accuracy and reduce bias in endothelial cell density (ECD) quantification by combining Gabor-domain optical coherence microscopy (GDOCM) for three-dimensional, wide field-of-view ( 1 mm2) corneal imaging and machine learning for automatic delineation of endothelial cell boundaries.
Corneal Scraping: Techniques and Diagnostic Applications
2024年7月23日 · Among the tools used by ophthalmologists, corneal scraping stands out as a vital procedure. This technique involves carefully removing cells from the corneal surface to allow for detailed laboratory analysis. Corneal scraping holds significant importance in identifying infectious organisms and other pathological entities affecting the eye.
Whole mount immunofluorescence analysis of fresh and stored …
2024年10月1日 · We utilized whole mount immunofluorescence (WM-IF) to evaluate how the storage affects the tissue integrity and putative limbal stem cells in human and porcine corneas. Moreover, we compare this information with the marker expression patterns observed in human pluripotent stem cell (hPSC)-derived LSCs.