YAP and TAZ protect against white adipocyte cell death during …
2020年10月28日 · Histological analysis of adipose tissue from HFD-fed Ad-Yap/Taz-KO mice showed increased numbers of TUNEL-positive adipocytes undergoing apoptosis as well as increased numbers of crown-like ...
Pioglitazone induces apoptosis of macrophages in human adipose …
Here, adipose tissue samples from IGT subjects treated with pioglitazone were examined for apoptosis with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. TUNEL-positive cells were identified, and there was a significant 42% increase in TUNEL-positive cells following pioglitazone treatment.
Adipocyte‐specific FAK deletion promotes pancreatic β‐cell …
2024年6月26日 · TUNEL staining results revealed a significant increase in TUNEL-positive cells within the FAK siRNA group (Figure 2F). Flow cytometry analysis demonstrated an elevated number of apoptotic adipocytes in the FAK siRNA group (Figure 2G).
Adipocyte Apoptosis, a Link between Obesity, Insulin Resistance, …
Adipose tissue apoptosis was determined by TUNEL assay, and macrophage infiltration was assessed by CD68 immunostaining. The percentage of TUNEL-positive cells and CD68-positive cells in adipose tissue were determined for each patient and related to the presence of insulin resistance syndrome (BMI, hypertension, hyperlipidemia, and glucose ...
BMPR2 promotes fatty acid oxidation and protects white
2020年4月29日 · BMPR2 deficiency in adipocytes inhibited phosphorylation of perilipin, a lipid-droplet-coating protein, and impaired lipolysis when stimulated by tumor necrosis factor (TNFα), which lead to...
Pioglitazone induces apoptosis of macrophages in human adipose …
2006年9月1日 · Here, adipose tissue samples from IGT subjects treated with pioglitazone were examined for apoptosis with terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) staining. TUNEL-positive cells were identified, and there was a significant 42% increase in TUNEL-positive cells following pioglitazone treatment.
High-Fat Diet–Induced Adipocyte Cell Death Occurs Through a …
2011年7月18日 · Adipose tissue from NCD-fed wild-type mice had a baseline level of nuclei positive for transferase-mediated dUTP nick-end labeling (TUNEL), whereas TUNEL-positive nuclei were significantly increased after 14 weeks of the HFD (Fig. 2A and B). Similarly, NCD-fed GM-CSF–null mice also had a low level of TUNEL-positive nuclei.
Anti-apoptotic effect of adipose tissue-derived stromal vascular ...
2021年3月4日 · Annexin V and TUNEL were used together with Western blotting to investigate the signaling pathways. Denervated muscles showed significantly higher TUNEL reactivity at 2 and 4 weeks following nerve injury, and an increased expression of caspase family genes, mitochondria-related apoptotic genes, and tumor necrosis factor family genes.
Preparation of Apoptotic Extracellular Vesicles from Adipose …
2023年6月2日 · TUNEL-stained images demonstrated apoptosis occurred in many cells of apoptotic adipose tissue, in contrast, no TUNEL-stained nuclei appeared in native adipose tissue (Figure 1C). To further evaluate the characteristics of ApoEVs-AT, the morphology, size distribution, and specific markers were analyzed.
JCI Insight - Increased apoptosis and browning of TAK1-deficient ...
2016年5月19日 · TUNEL assays in adipose tissue sections from wild-type and AdTAK1-KO mice revealed more TUNEL-positive cells in the adipose tissue of AdTAK1-KO mice . We then determined caspase-3 activity in freshly isolated adipocytes from wild-type and AdTAK1-KO mice fed with chow or a high-fat diet (HFD) or on a leptin-deficient background (ob/ob